Multiscale modelling of biomolecular corona formation on metallic surfaces

• Parinaz Mosaddeghi Amini,
• Ian Rouse,
• Julia Subbotina and
• Vladimir Lobaskin

Beilstein J. Nanotechnol. 2024, 15, 215–229, doi:10.3762/bjnano.15.21

• reactivity, sensitivity of NPs, as well as their cellular uptake and systemic transfer [21]. However, in order to develop predictive models, a deeper understanding of the interactions at the bionano interface and their relationship to material and protein properties is necessary. Gathering more information

Nanocarrier systems loaded with IR780, iron oxide nanoparticles and chlorambucil for cancer theragnostics

• Phuong-Thao Dang-Luong,
• Hong-Phuc Nguyen,
• Loc Le-Tuan,
• Xuan-Thang Cao,
• Vy Tran-Anh and
• Hieu Vu Quang

Beilstein J. Nanotechnol. 2024, 15, 180–189, doi:10.3762/bjnano.15.17

• and F127@NP had the same impact. However, both NPs produced superior outcomes than those for PVA@NP control. It has been demonstrated that F127 can enhance the cellular uptake of NPs in vitro via clathrin-mediated endocytosis and caveolae-mediated endocytosis [31][40]. The absorption of F127 onto

Curcumin-loaded nanostructured systems for treatment of leishmaniasis: a review

• Douglas Dourado,
• Thayse Silva Medeiros,
• Éverton do Nascimento Alencar,
• Edijane Matos Sales and
• Fábio Rocha Formiga

Beilstein J. Nanotechnol. 2024, 15, 37–50, doi:10.3762/bjnano.15.4

• context, we could observe that the studies failed to correlate consecutive intra-macrophage and intra-amastigote cellular uptake kinetics, once it appears to greatly interfere with the proposed biochemical triggers of Leishmania spp. cell death. In addition, the work could also summarize that lipid-based

Elasticity, an often-overseen parameter in the development of nanoscale drug delivery systems

• Agnes-Valencia Weiss and
• Marc Schneider

Beilstein J. Nanotechnol. 2023, 14, 1149–1156, doi:10.3762/bjnano.14.95

• examining the characteristics of nanoparticles used for drug delivery, one can see that some are better understood then others. The size of nanoparticles, for example, is shown to play an important role in tissue or mucus penetration [8] and in cellular uptake [9]. Also surface charge and chemical

• properties are well investigated regarding their effect on cellular uptake and the influence on in vivo performance [3][10]. Only since the first decade of the 2000s, mechanical properties have been investigated in the development of nanoparticulate drug delivery systems. A well-known example in biology

• particles showed better cellular uptake if no mucus layer was present. In contrast to this, the cellular uptake for semielastic particles was not significantly affected by the presence of a mucus layer [38]. Liposomes with PLGA cores were used by Yu et al. to increase the stiffness in combination with

Curcumin-loaded albumin submicron particles with potential as a cancer therapy: an in vitro study

• Nittiya Suwannasom,
• Netsai Sriaksorn,
• Chutamas Thepmalee,
• Krissana Khoothiam,
• Ausanai Prapan,
• Hans Bäumler and
• Chonthida Thephinlap

Beilstein J. Nanotechnol. 2023, 14, 1127–1140, doi:10.3762/bjnano.14.93

• silk core–shell nanoparticles show high cytotoxicity and cellular uptake regarding breast cancer cells [14]. However, the effectiveness of zein nanoparticles as a delivery vehicle is limited by their poor stability, as they tend to aggregate when suspended in water [15]. Lyophilizing the particles

• cellular uptake of HSA-MPs and CUR-HSA-MPs. The intrinsic fluorescence of both particle types excited at 488 nm allowed for their visualization, as the fluorescence properties were preserved upon encapsulation (Figure 2E). Quantitative analysis of cellular uptake, performed through flow cytometry, revealed

• -MPs by MCF-7 cells is challenging. Cellular uptake was measured in MCF-7 cells as these cells have a high affinity for albumins. Figure 8C shows a z-stack section for FITC-CUR-HSA-MPs, exhibiting green fluorescence due to the uptake of CUR. This demonstrates that CUR-HSA-MPs were readily taken up by

Antibody-conjugated nanoparticles for target-specific drug delivery of chemotherapeutics

• Mamta Kumari,
• Amitabha Acharya and
• Praveen Thaggikuppe Krishnamurthy

Beilstein J. Nanotechnol. 2023, 14, 912–926, doi:10.3762/bjnano.14.75

• fragment antigen-binding (Fab) region of mAbs are chemically conjugated to NP surfaces to recognize protein targets that are overexpressed on the surface of tumor cells. Conjugation of mAbs to NP surfaces improves targeting capacity, cellular uptake, and intracellular stability [12]. The mAb-functionalized

• Herceptin® to improve cellular uptake and cytotoxicity in breast cancer cells [41]. Similarly, Rayavarapu et al. conjugated HER2 antibodies on the surface of gold nanoparticles using a noncovalent conjugation method in order to increase intracellular uptake into cancer cells [42]. The adsorption results

• of the specific nature of the targeting antibody. ACNPs are expected to accumulate at the target site and to improve the tumor uptake. ACNPs receptor binding efficacies were determined using in vitro studies including cellular uptake and internalization studies [71]. Multivalent effect of antibody

Nanostructured lipid carriers containing benznidazole: physicochemical, biopharmaceutical and cellular in vitro studies

• Giuliana Muraca,
• María Esperanza Ruiz,
• Rocío C. Gambaro,
• Sebastián Scioli-Montoto,
• María Laura Sbaraglini,
• Gisel Padula,
• José Sebastián Cisneros,
• Cecilia Yamil Chain,
• Vera A. Álvarez,
• Cristián Huck-Iriart,
• Guillermo R. Castro,
• María Belén Piñero,
• Matias Ildebrando Marchetto,
• Catalina Alba Soto,
• Germán A. Islan and
• Alan Talevi

Beilstein J. Nanotechnol. 2023, 14, 804–818, doi:10.3762/bjnano.14.66

• high-negative ζ values may impede cellular uptake [40]. On the other hand, it was observed that the nanoparticulated systems remained stable after six months with no precipitation. This suggests that in this case the stabilization is not achieved by means of surface charge alone, but also by the steric

Carboxylic acids and light interact to affect nanoceria stability and dissolution in acidic aqueous environments

• Matthew L. Hancock,
• Eric A. Grulke and
• Robert A. Yokel

Beilstein J. Nanotechnol. 2023, 14, 762–780, doi:10.3762/bjnano.14.63

• , distribution, and toxicity of nanoceria within biological systems. Cellular uptake studies of nanoceria in lung adenocarcinoma (A549) cells favored particles with a negative zeta potential. However, positively charged particles resulted in greater bovine serum albumin adsorption. This suggests that surface

The steep road to nonviral nanomedicines: Frequent challenges and culprits in designing nanoparticles for gene therapy

• Yao Yao,
• Yeongun Ko,
• Grant Grasman,
• Jeffery E. Raymond and
• Joerg Lahann

Beilstein J. Nanotechnol. 2023, 14, 351–361, doi:10.3762/bjnano.14.30

• Need for Multimodal Characterization of Nanoparticles The methods chosen to investigate NP uptake and transfection can be biased towards particular properties and may provide limited insights into the efficiency of NP internalization and efficacy. Typically, cellular uptake and transfection efficiency

• ]. This technology measures the mean fluorescence intensity and percent positive value of cells, while also capturing an image of each individual event; this provides information, such as cellular distribution patterns of NPs [22], while also allowing for investigating cellular uptake pathways and

• 50 nanoparticle (NP)-mediated plasmid DNA (pDNA) delivery experiments published in 2017–2022. (a) 5-year prevalence of reporting imaging, imaging quantification, and 3D imaging capture for investigating NP cellular uptake and (or) transfection. (b) 5-year prevalence of reporting flow cytometry for

Polymer nanoparticles from low-energy nanoemulsions for biomedical applications

• Santiago Grijalvo and
• Carlos Rodriguez-Abreu

Beilstein J. Nanotechnol. 2023, 14, 339–350, doi:10.3762/bjnano.14.29

• corona formation (upon incubation in FBS). The nanoparticles showed low cytotoxicity for SH-SY5Y cells (viabilities of ca. 100% for nanoparticle concentrations equal or lower than 0.23 mg/mL), high cellular uptake (in SH-SY5Y cells), and dose-dependent antioxidant activity. The properties of the PIC

Structural, optical, and bioimaging characterization of carbon quantum dots solvothermally synthesized from o-phenylenediamine

• Zoran M. Marković,
• Milica D. Budimir,
• Martin Danko,
• Dušan D. Milivojević,
• Pavel Kubat,
• Danica Z. Zmejkoski,
• Vladimir B. Pavlović,
• Marija M. Mojsin,
• Milena J. Stevanović and
• Biljana M. Todorović Marković

Beilstein J. Nanotechnol. 2023, 14, 165–174, doi:10.3762/bjnano.14.17

• oxygen species production, and showed low dark cytotoxicity. By investigating the cellular uptake, it was established that these dots penetrated the HeLa cells and could be used as probes for bioimaging. Keywords: antibacterial; bioimaging; carbon quantum dots; precursor; reactive oxygen species

• composites has a crucial effect on the properties of CQDs/PU composites. Cellular uptake Photobleaching limits the use of hydrophobic probes (e.g., Nile red) [50]. CQDs can be used as probes for bioimaging because of the tuneable strong photoluminescence and high resistance to photobleaching. In order to

Facile preparation of Au- and BODIPY-grafted lipid nanoparticles for synergized photothermal therapy

• Yuran Wang,
• Xudong Li,
• Haijun Chen and
• Yu Gao

Beilstein J. Nanotechnol. 2022, 13, 1432–1444, doi:10.3762/bjnano.13.118

• could be associated stably to Au-LNPs, and the release of BODIPY from AB-LNPs could be accelerated by laser irradiation. AB-LNPs are scalable and showed excellent photothermal effects. AB-LNPs showed enhanced cellular uptake efficiency compared to free BODIPY in 4T1 breast cancer cells. Under laser

• -LNPs with optimized particle size and polydispersity index (PDI), and then mixed BDP with Au-LNPs to obtain both Au- and BDP-grafted LNPs (AB-LNPs). The physiochemical characteristics, the photothermal properties, and the stabilities of AB-LNPs were studied in detail. The cellular uptake efficiency and

• 10% FBS. The cells were cultured in a humidified incubator with atmosphere of 5% CO2 at 37 °C. Cellular uptake studies 4T1 cells were grown in 12-well plates and cultured for 24 h. The cells were incubated with free BDP (30 μM) and AB-LNPs (with a BDP concentration of 30 μM and a Au concentration of

Orally administered docetaxel-loaded chitosan-decorated cationic PLGA nanoparticles for intestinal tumors: formulation, comprehensive in vitro characterization, and release kinetics

• Sedat Ünal,
• Osman Doğan and
• Yeşim Aktaş

Beilstein J. Nanotechnol. 2022, 13, 1393–1407, doi:10.3762/bjnano.13.115

• carboxyl groups of PLGA at the NP surface [43]. CS is a cationic heteropolysaccharide, which promotes cellular uptake, mucoadhesiveness, and tissue penetration [42]. Therefore, CS interacts easily with negatively charged groups at the surface of the NPs and increases the tissue penetration. Morphology of

• DCX-loaded nanoparticles Morphological properties of NPs are among the most important factors influencing the efficacy of drugs and determining the fate of NP systems. It has been reported that the NP morphology significantly affects circulation time and cellular uptake of NPs [44]. Morphological

• concentration (p < 0.05). Additionally, CS/PLGA NPs exhibited a higher anticancer activity than DCX-PLGA formulations (p < 0.05). It is suggested that this is related to the positively charged surface of the CS-coated PLGA NPs and a strong cellular interaction resulting in increased cellular uptake. In the

Design and selection of peptides to block the SARS-CoV-2 receptor binding domain by molecular docking

• Kendra Ramirez-Acosta,
• Ivan A. Rosales-Fuerte,
• J. Eduardo Perez-Sanchez,
• Alfredo Nuñez-Rivera,
• Josue Juarez and
• Ruben D. Cadena-Nava

Beilstein J. Nanotechnol. 2022, 13, 699–711, doi:10.3762/bjnano.13.62

• previously by Qiao & Olvera, who designed a negatively charged EELE tetrapeptide to neutralize the SARS-CoV-2-RBD–ACE2 binding [25]. It is important to note that the analyzed peptides have a nearly neutral charge, thus they have a low probability of unspecific interactions with other molecules, cellular

• uptake, or macrophage recognition [42][43][44][45][46]. It is important to note that RBD residues from Glu484 to Tyr505, Arg403 to Tyr421, and Tyr449 to Ala475 are involved in the docking of the APD peptides, as was previously reported by Othman and co-workers [6]. Figure 2 shows the mapping of these

Detection and imaging of Hg(II) in vivo using glutathione-functionalized gold nanoparticles

• Gufeng Li,
• Shaoqing Li,
• Rui Wang,
• Min Yang,
• Lizhu Zhang,
• Yanli Zhang,
• Wenrong Yang and
• Hongbin Wang

Beilstein J. Nanotechnol. 2022, 13, 549–559, doi:10.3762/bjnano.13.46

• living cells was observed gradually (Figure 6b and Figure 6e), indicating that GSH-Rh6G2 and GNPs-GSH-Rh6G2 could enter the cells and that the release of RGCOOH was triggered by intracellular Hg2+. Importantly, we found that the cellular uptake of GNPs-GSH-Rh6G2 was higher than that of GSH-Rh6G2. This

Systematic studies into uniform synthetic protein nanoparticles

• Nahal Habibi,
• Ava Mauser,
• Jeffery E. Raymond and
• Joerg Lahann

Beilstein J. Nanotechnol. 2022, 13, 274–283, doi:10.3762/bjnano.13.22

• response at the cellular level. Several studies [28][29][30] have shown that considerations such as aspect ratio, stiffness/deformability, and particle surface roughness (deviation of circularity) can have a comparable impact on cellular uptake and/or endosomal escape. Hence, it is important to incorporate

Engineered titania nanomaterials in advanced clinical applications

• Padmavati Sahare,
• Paulina Govea Alvarez,
• Juan Manual Sanchez Yanez,
• Gabriel Luna-Bárcenas,
• Samik Chakraborty,
• Sujay Paul and
• Miriam Estevez

Beilstein J. Nanotechnol. 2022, 13, 201–218, doi:10.3762/bjnano.13.15

• -functionalized, or post-synthetically modified by adding various surfactants or dopants or organic molecules. The size of the nanomaterial also determines the type of immune response elicited by the body (endocytosis/cellular uptake) [21]. Xu et al. reported that the size of the pores is an essential parameter

• cervical tumors by incorporating zinc phthalocyanine (ZnPc) as photosensitizer into TiO2 nps. The result showed a higher cellular uptake of ZnPc-TiO2 and an increased PDT efficiency compared ot Zn alone [114]. Since photocatalytic absorption generally occurs at the surface, surface modification acts as the

Self-assembly of amino acids toward functional biomaterials

• Huan Ren,
• Lifang Wu,
• Lina Tan,
• Yanni Bao,
• Yuchen Ma,
• Yong Jin and
• Qianli Zou

Beilstein J. Nanotechnol. 2021, 12, 1140–1150, doi:10.3762/bjnano.12.85

• time. However, a weak fluorescence signal was observed in the tumor site of the mice treated with free Ce6. Designing nanoplatforms responsive to pH, enzymes, and photothermal stimuli is critical to enhance cellular uptake and control PS release [79][80][81]. Nanoplatforms responsive to pH undergo

• conformational changes through various mechanisms, such as protonation, charge inversion, or chemical bond cleavage, promoting tumor-specific cellular uptake or drug release [82]. Sun et al. [83] coupled tryptophan-glycine (WG) to hydrophobic porphyrins (P) by an amidation reaction to obtain pH-responsive

• linked by ester bonds, which help to maintain the camptothecin ring stability. The assembly can effectively enhance blood circulation, tumor accumulation and cellular uptake. In addition, arginine-modified camptothecin can be combined with anionic cisplatin–polyglutamic acid through electrostatic

pH-driven enhancement of anti-tubercular drug loading on iron oxide nanoparticles for drug delivery in macrophages

• Karishma Berta Cotta,
• Sarika Mehra and
• Rajdip Bandyopadhyaya

Beilstein J. Nanotechnol. 2021, 12, 1127–1139, doi:10.3762/bjnano.12.84

• antibiotics of this class, like ciprofloxacin and moxifloxacin. The quantification of the intra-macrophage accumulation of NOR shows that, cellular uptake is greatly facilitated by drug coated systems too, compared to free drug. Although both drug-coated nanoparticle systems enhance the drug uptake, due to

Use of nanosystems to improve the anticancer effects of curcumin

• Andrea M. Araya-Sibaja,
• Norma J. Salazar-López,
• Krissia Wilhelm Romero,
• José R. Vega-Baudrit,
• J. Abraham Domínguez-Avila,
• Carlos A. Velázquez Contreras,
• Ramón E. Robles-Zepeda,
• Mirtha Navarro-Hoyos and
• Gustavo A. González-Aguilar

Beilstein J. Nanotechnol. 2021, 12, 1047–1062, doi:10.3762/bjnano.12.78

• characteristic of the tumor microenvironment (pH 6.5) and subcellular endosomes (pH 5.0). Regarding its apoptotic effects, a high anticancer activity was found when assayed against a breast cancer cell line (MDA-MB-231), an effect that was accompanied by an enhanced cellular uptake in cells that overexpressed

• the expulsion of the bioactive compound and, therefore, a rapid initial release [66]. Curcumin-loaded SLN were tested in vitro against MDA-MB-231 cells, which revealed a significantly higher cytotoxicity, cellular uptake, and induced apoptosis, as compared to F-CUR [67]. Curcumin-based SLN have shown

• receptors), while the enhanced effect of permeation and retention was considered for passive targeting. Results showed improved in vitro cellular uptake, targeting capability, and cytotoxicity against the human colon cancer cell line HCT116, which was related to early apoptosis after 24 h of incubation. On

Comprehensive review on ultrasound-responsive theranostic nanomaterials: mechanisms, structures and medical applications

• Sepand Tehrani Fateh,
• Lida Moradi,
• Elmira Kohan,
• Michael R. Hamblin and
• Amin Shiralizadeh Dezfuli

Beilstein J. Nanotechnol. 2021, 12, 808–862, doi:10.3762/bjnano.12.64

• become subjected to cavitation, thus promoting cellular uptake and delivery of entrapped drug/agents into the desired area [127]. Acoustic droplet vaporization has been carried out with many liquids whose boiling points are close to the body temperature. Fluorocarbons, especially perfluorocarbons (PFCs

Fate and transformation of silver nanoparticles in different biological conditions

• Barbara Pem,
• Marija Ćurlin,
• Darija Domazet Jurašin,
• Valerije Vrček,
• Rinea Barbir,
• Vedran Micek,
• Raluca M. Fratila,
• Jesus M. de la Fuente and
• Ivana Vinković Vrček

Beilstein J. Nanotechnol. 2021, 12, 665–679, doi:10.3762/bjnano.12.53

• Table 1) after 0, 1, 4, and 24 h of incubation. The media AGF, ALF, and CCM can be used to evaluate the AgNP transformation during their passage from the gastrointestinal system into the body, after entering the extracellular matrix and going through cellular uptake. Furthermore, the behaviour of AgNPs

The impact of molecular tumor profiling on the design strategies for targeting myeloid leukemia and EGFR/CD44-positive solid tumors

• Nikola Geskovski,
• Nadica Matevska-Geshkovska,
• Simona Dimchevska Sazdovska,
• Marija Glavas Dodov,
• Kristina Mladenovska and
• Katerina Goracinova

Beilstein J. Nanotechnol. 2021, 12, 375–401, doi:10.3762/bjnano.12.31

Characterization, bio-uptake and toxicity of polymer-coated silver nanoparticles and their interaction with human peripheral blood mononuclear cells

• Sahar Pourhoseini,
• Reilly T. Enos,
• Angela E. Murphy,
• Bo Cai and
• Jamie R. Lead

Beilstein J. Nanotechnol. 2021, 12, 282–294, doi:10.3762/bjnano.12.23

• . Mass balance data for each person after AgNP and AgNO3 treatments are presented in Table S1 (Supporting Information File 1). At concentrations of 10, 100, 500 and 1000 µg·L−1 PVP-AgNPs, the ratio between cellular uptake and content in the supernatant did not show any significant increase or trend

• strongly to the cells) after AgNO3 treatment compared to NP treatment at concentrations of 500 and 1000 µg·L−1, which is consistent with findings from previous studies [28]. The higher cellular uptake of Ag in the form of ions is possibly related to a higher uptake rate for dissolved Ag [51] or because of

• the effect of cell type and the kinetics of cellular uptake or sorption [40]. Human PBMCs primarily consist of monocytes and lymphocytes (mostly T cells). Monocytes are known for their phagocytic properties and phagocytosis is suggested as one of the possible mechanisms for Ag uptake by cells [7]. As

Differences in surface chemistry of iron oxide nanoparticles result in different routes of internalization

• Barbora Svitkova,
• Vlasta Zavisova,
• Veronika Nemethova,
• Martina Koneracka,
• Miroslava Kretova,
• Filip Razga,
• Monika Ursinyova and
• Alena Gabelova

Beilstein J. Nanotechnol. 2021, 12, 270–281, doi:10.3762/bjnano.12.22

• involved in the internalization of polyethylene glycol-coated MNPs. Our data indicate that surface engineering can contribute to an enhanced delivery efficiency of nanoparticles. Keywords: bovine serum albumin; cellular uptake; magnetic iron oxide nanoparticles; polyethylene glycol; surface coating

• cellular uptake of nanoparticles. Therefore, initial experiments were focused on the expression of clathrin heavy chain (CLHC), dynamin (Dyn), caveolin-1 (Cav1), and its phosphorylated form (pCav1) in A549 cells. The expression of CLHC and Cav1 was determined at the protein (Supporting Information File 1

• distinct pathway(s) underlying cellular uptake and the parameters influencing this process is of great importance for the design of new tailored nanovectors for different cells/tissues in biomedical applications. It is well documented that the physicochemical parameters of nanoparticles substantially